Studies on cultured Schwann cells: the induction of myelin synthesis, and the control of their proliferation by a new growth factor

We have recently described the use of immunological methods to identify and purify rat Schwann cells. In dissociated cultures of neonatal sciatic nerve, all of the cells can be identified by antigenic criteria as either Schwann cells or fibroblasts. The fibroblasts may be removed by treatment with antiserum to the Thy-1 antigen and complement. The purified Schwann cells have been used to study the regulation of the expression of myelin components, and the stimulation of Schwann cell division by a soluble growth factor. Among the components of myelin, we have concentrated on the peripheral myelin glycoprotein P_0, which constitutes 50–60% of the protein in peripheral myelin. We have studied the distribution of P_0 in vitro and in vivo by immunofluorescence, immuno-autoradiography on SDS gels, and solid-phase radioimmunoassay. Our results support the hypothesis that P_0 is induced specifically as a consequence of the interaction between the Schwann cell and the myelinated type of axon. The level of P_0 in the myelin membrane is at least 1000-fold higher than in the Schwann cell membrane. Purified Schwann cells divide very slowly in a conventional tissue culture medium. This has allowed us to purify a new growth factor from extracts of brain and pituitary, tentatively named Glial Growth Factor (GGF). The activity resides in a basic protein with a native molecular weight of 6 × 10^4 daltons and a subunit molecular weight of 3 × 10^4 daltons, which is active at levels comparable to those of epidermal growth factor. GGF is mitogenic for Schwann cells, astrocytes and muscle fibroblasts

Simulations of Astrophysical Fluid Instabilities

We present direct numerical simulations of mixing at Rayleigh-Taylor unstable interfaces performed with the FLASH code, developed at the ASCI/Alliances Center for Astrophysical Thermonuclear Flashes at the University of Chicago. We present initial results of single-mode studies in two and three dimensions. Our results indicate that three-dimensional instabilities grow significantly faster than two-dimensional instabilities and that grid resolution can have a significant effect on instability growth rates. We also find that unphysical diffusive mixing occurs at the fluid interface, particularly in poorly resolved simulations.Comment: 3 pages, 1 figure. To appear in the proceedings of the 20th Texas Symposium on Relativistic Astrophysic

Large-Scale Simulations of Clusters of Galaxies

We discuss some of the computational challenges encountered in simulating the evolution of clusters of galaxies. Eulerian adaptive mesh refinement (AMR) techniques can successfully address these challenges but are currently being used by only a few groups. We describe our publicly available AMR code, FLASH, which uses an object-oriented framework to manage its AMR library, physics modules, and automated verification. We outline the development of the FLASH framework to include collisionless particles, permitting it to be used for cluster simulation.Comment: 3 pages, 3 figures, to appear in Proceedings of the VII International Workshop on Advanced Computing and Analysis Techniques in Physics Research (ACAT 2000), Fermilab, Oct. 16-20, 200

Asynchronous food-web pathways could buffer the response of Serengeti predators to El Niño southern oscillation

Understanding how entire ecosystems maintain stability in the face of climatic and human disturbance is one of the most fundamental challenges in ecology. Theory suggests that a crucial factor determining the degree of ecosystem stability is simply the degree of synchrony with which different species in ecological food webs respond to environmental stochasticity. Ecosystems in which all food-web pathways are affected similarly by external disturbance should amplify variability in top carnivore abundance over time due to population interactions, whereas ecosystems in which a large fraction of pathways are nonresponsive or even inversely responsive to external disturbance will have more constant levels of abundance at upper trophic levels. To test the mechanism underlying this hypothesis, we used over half a century of demographic data for multiple species in the Serengeti (Tanzania) ecosystem to measure the degree of synchrony to variation imposed by an external environmental driver, the El Niño Southern Oscillation (ENSO). ENSO effects were mediated largely via changes in dry-season vs. wet-season rainfall and consequent changes in vegetation availability, propagating via bottom-up effects to higher levels of the Serengeti food web to influence herbivores, predators and parasites. Some species in the Serengeti food web responded to the influence of ENSO in opposite ways, whereas other species were insensitive to variation in ENSO. Although far from conclusive, our results suggest that a diffuse mixture of herbivore responses could help buffer top carnivores, such as Serengeti lions, from variability in climate. Future global climate changes that favor some pathways over others, however, could alter the effectiveness of such processes in the future

Circinus X-1: survivor of a highly asymmetric supernova

We have analyzed the kinematical parameters of Cir X-1 to constrain the nature of its companion star, the eccentricity of the binary and the pre-supernova parameter space. We argue that the companion is most likely to be a low-mass (< 2.0 M_sun) unevolved star and that the eccentricity of the orbit is 0.94 +/- 0.04. We have evaluated the dynamical effects of the supernova explosion and we find it must have been asymmetric. On average, we find that a kick of 740 km/s is needed to account for the recently measured radial velocity of +430 km/s (Johnston, Fender & Wu) for this extreme system. The corresponding minimum kick velocity is 500 km/s. This is the largest kick needed to explain the motion of any observed binary system. If Cir X-1 is associated with the supernova remnant G321.9-0.3 then we find a limiting minimum age of this remnant of 60000 yr. Furthermore, we predict that the companion star has lost 10% of its mass as a result of stripping and ablation from the impact of the supernova shell shortly after the explosion.Comment: 6 pages, 3 figues, 2 tables, accepted for publication in MNRA

Ionization front-driven turbulence in the clumpy interstellar medium

We present 3D radiation-gasdynamical simulations of an ionization front running into a dense clump. In our setup, a B0 star irradiates an overdensity which is at a distance of 10 pc and modelled as a supercritical 100 M_sol Bonnor-Ebert sphere. The radiation from the star heats up the gas and creates a shock front that expands into the interstellar medium. The shock compresses the clump material while the ionizing radiation heats it up. The outcome of this "cloud-crushing" process is a fully turbulent gas in the wake of the clump. In the end, the clump entirely dissolves. We propose that this mechanism is very efficient in creating short-living supersonic turbulence in the vicinity of massive stars

Onset of Convectionon a Pre-Runaway White Dwarf

Observed novae abundances and explosion energies estimated from observations indicate that there must be significant mixing of the heavier material of the white dwarf (C+O) into the lighter accreted material (H+He). Accordingly, nova models must incorporate a mechanism that will dredge up the heavier white dwarf material, and fluid motions from an early convection phase is one proposed mechanism. We present results from two-dimensional simulations of classical nova precursor models that demonstrate the beginning of a convective phase during the `simmering' of a Nova precursor. We use a new hydrostatic equilibrium hydrodynamics module recently developed for the adaptive-mesh code FLASH. The two-dimensional models are based on the one-dimensional models of Ami Glasner, and were evolved with FLASH from a pre-convective state to the onset of convection.Comment: 5 pages, 4 figures, from the 2002 International Conference on Classical Novae in Sitges, Spai

Mixing by Non-linear Gravity Wave Breaking on a White Dwarf Surface

We present the results of a simulation of a wind-driven non-linear gravity wave breaking on the surface of a white dwarf. The ``wind'' consists of H/He from an accreted envelope, and the simulation demonstrates that this breaking wave mechanism can produce a well-mixed layer of H/He with C/O from the white dwarf above the surface. Material from this mixed layer may then be transported throughout the accreted envelope by convection, which would enrich the C/O abundance of the envelope as is expected from observations of novae.Comment: 5 pages, 3 figures, to appear in the proceedings of the International Conference on Classical Nova Explosions, Sitges, Spain, 20-24 May 200